Upregulation of AIFM2 was further confirmed at mRNA level by quantitative reverse transcription PCR (qRT-PCR) analysis in 30-paired tumor and adjacent non-tumor tissue samples (Fig. 1C) and at protein level by immunohistochemistry (IHC) analysis in another cohort of 213-paired tumor and adjacent non-tumor tissue samples (Fig. 1D). The gene discussed is AIFM2; the disease is neoplasm.