To determine whether ZIKV infection affects hormone secretion of the HPG axis, we used enzyme-linked immunosorbent assay (ELISA) to assay serum concentration of four hormones, including gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone. This evidence concerns the gene BRD2 and Zika virus infectious disease.