The results showed that the expression of ASNS (p = 0.0036), CD70 (p = 0.019) and TRIB3 (p = 0.069) expression was upregulated while SRGN (p = 0.079) expression was slightly downregulated in osteosarcoma samples compared with normal samples, which was consistent with the findings in the training dataset. The gene discussed is TRIB3; the disease is osteosarcoma.