Conforming to the ROC curves in Figure 6A, the results were as follows: ASNS (AUC 0.928, 95% CI 0.837–0.987), SRGN (AUC 0.954, 95% CI 0.867–1.000), CD70 (AUC 0.900, 95% CI 0.814–0.964), TRIB3 (AUC 0.955, 95% CI 0.900–0.991), indicating that all 4 genes had high AUC value than 0.90 and possessed excellent diagnostic value for osteosarcoma patients (Figure 6A). This evidence concerns the gene TRIB3 and osteosarcoma.