A recent analysis investigated the PAX7 and DUX4 target gene biomarker sets in FSHD PBMCs via the ‘globaltest’ package.31 Unlike our approach, ‘globaltest’ does not give a single sample score for use as a biomarker but determines the association between a set of genes and given phenotype. The gene discussed is DUX4; the disease is facioscapulohumeral muscular dystrophy.