First, we created a 7-plex immunofluorescent staining panel to be used on a colorectal tumor tissue (CRC1) consisting of a tumor marker (EPCAM), T-cell markers (CD3, CD4, and CD8), and myeloid markers (CD163, HLA-DR, and XCR1) (Figure 6A). This evidence concerns the gene CD8A and colorectal neoplasm.