TLR5 and malignant colon neoplasm: To exclude the possibility that TLR5 expression in colon cancer cells might affect the antitumor activity of CRC treatment, we performed IHC staining of TLR5 in human normal colon and primary colon cancer tissues and showed that TLR5 had a lower level of expression in primary colon tumor and normal colon tissues (Supplementary Figure S9D).