To ascertain if GSDMD and GSDME are required for IL-1β secretion following infection with ExoS expressing P. aeruginosa, bone marrow neutrophils isolated from Gsdmd-/-, Gsdme-/- and Gsdmd-/-/Gsdme-/- mice were primed with LPS and incubated 1 h with ATP, PAO1, ∆pscd or ∆exoST mutant bacteria. This evidence concerns the gene GSDMD and infection.