In agreement with the Sytox data, we found that up to 80% neutrophils were H3Cit+ following stimulation with PMA or infection with P. aeruginosa strain PAO1; however, there was no significant difference in H3Cit between neutrophils from C57BL/6 and Gsdmd-/- mice (Fig. 5I, J, Fig. S4C). This evidence concerns the gene GSDMD and infection.