GSDMD was cleaved in neutrophils and macrophages following infection with PAO1 (Fig. 1E). Caspase 3 and GSDME were also cleaved in response to TNF/SM in neutrophils and macrophages and there was no effect of TNF/CHX; however, PAO1 infection resulted in GSDME processing in neutrophils, but not in macrophages (Fig. 1E, F). This evidence concerns the gene TNF and infection.