However, although caspase-8 was cleaved in TNF/SM treated macrophages and neutrophils, caspase-8 was not cleaved following PAO1 infection (Fig. S1E), indicating that there is no requirement for caspase-8 in GSDMD processing or IL-1β secretion induced by PAO1 infection of neutrophils. This evidence concerns the gene CASP8 and infection.