In the routine diagnosis of HER2 expression, breast cancer tissue sections are stained with hematoxylin and eosin (H&E), the morphology is determined by manual visual inspection, and the expression levels of HER2-specific proteins are then measured by immunohistochemical (IHC) analysis and in situ hybridization (ISH) technology [11]. This evidence concerns the gene ERBB2 and breast carcinoma.