Specifically, the seminal mechanobiology studies have established that adhesion and crawling of mesenchymal cells along the 1D adhesion cues, such as bundled, anisotropically aligned collagen fibers or micropatterns of isolated adhesion lanes, often represent a special case of cell migration that generally does not critically require actomyosin contractility.[13, 28, 29, 30] Thus, we conclude that 2D crisscrossed collagen rhomboid grids are better suited for deciphering the contributing roles of myosin and dynein motors in the migration of metastatic breast cancer cells. The gene discussed is MYH14; the disease is breast carcinoma.