Our findings of increased LC3-II and p62 puncta in uninfected MDA5-KO cells over NTC (Figure 4B), and a decrease in IL-1β, IL-6, and TNF-α in uninfected MDA5-KO cells over NTC (Figure 6, E–G), suggest that MDA5 might have a role in modulating IFN-γ signaling not specific to M. tuberculosis infection, and this could have broader implications for other bacterial and viral infections. Here, TNF is linked to viral infectious disease.