MC4R and metabolic dysfunction-associated steatohepatitis: In this study, we focused on the lysosomal accumulation of free cholesterol and subsequent lysosomal dysfunction in CLS-constituting macrophages in our NASH model using genetically obese melanocortin 4 receptor–deficient (MC4R-KO) mice (Farrell et al., 2019; Itoh et al., 2011).