SPP1 and metabolic dysfunction-associated steatohepatitis: Several inflammatory cytokines and lysosome-related genes (Atp6v0d2) were similarly upregulated by the treatment with cholesterol crystals in the macrophages from normal and steatotic livers (cluster E), whereas genes characteristic for scar-associated macrophage (SAM) or NASH-associated macrophage (Ramachandran et al., 2019; Xiong et al., 2019), such as Itgax, Vegfa, Fabp4, Spp1, and Kcnn4, were upregulated only in the macrophages from steatotic livers (clusters B and C).