SMAD2 and Bloom syndrome: In addition, western blotting (Figure 7E, 7F) and immunofluorescence staining (Figure 7A, 7C) suggested that the phosphorylation level of Smad2/3 was increased in BLM-induced Fl/Fl mice compared with saline-injected Fl/Fl mice, but significantly decreased the p-Smad2/3 expression was observed in BLM-induced CKO mice compared with BLM-induced Fl/Fl mice.