These quantitative biophysical and imaging data indicate that wild-type 2N4R mouse tau expressed predominantly in matured cultured neurons can be misfolded and aggregated with various rates when exposed to human AD-tau and that individual AD-tau with distinct conformational characteristics trigger the formation of predominantly 4-four-repeat mouse tau conformers with homologous initial characteristics which are different from case to case. Here, MAPT is linked to Alzheimer disease.