We also detected the changes in Treg cells in the subcutaneous tumor-bearing model, and the results showed that LFA-1 knockout decreased the numbers of CD4 + CD25 + Foxp3 + Treg cells in the blood, spleens, and lymph nodes (Fig. 3D-F H-J; **P < 0.01, *P < 0.05). This evidence concerns the gene ITGAL and neoplasm.