The frequency of simultaneous transcription of TLR7 and TLR8 was again sevenfold greater on the Xa of women (group mean 31%) than on the X of XY men (4.1%; 95% CI 2.4–6.0%), while the overall frequency of positive events (for TLR7, TLR8, or both genes together) was similar between women, normal men and men with Klinefelter syndrome (Additional file 2: Fig S5E). This evidence concerns the gene TLR8 and Klinefelter syndrome.