AGO2 and infection: Arbovirus infection also caused the mortality of ArgoN−/− mutants, a mutation in the ArgoN domain of Ago2, but it is lower than that of Ago2−/− mutants (Supplementary Fig. 5b); these results were correlated with the virus titer in the various Ago2 knockout mutant lines (Fig. 1d), suggesting that the high mortality of the Ago2−/− mutants may be a result of their high infection intensity.