AGO2 and arbovirus infection: Hence, a mutation in the Piwi_ago-like domain had the strongest impact on arbovirus infection and Ago2 endonuclease activity, which was also shown in dsRNA-mediated silencing of endogenous genes (Supplementary Fig. 1d), and the Ago2−/− line was therefore used as a Ago2 mutant line for the subsequent experiments.