In the present study, we were surprised to find that KLF4 expression was significantly positively correlated with the expression of myogenic regulators Myog, MyoD, Myf5, and Myf6 in healthy human skeletal muscle samples of various ages, and KLF4 expression was upregulated in the skeletal muscle of Duchenne muscular dystrophy (DMD) mice. The gene discussed is MYOG; the disease is Duchenne muscular dystrophy.