Earlier, the modelof inflammation in the tumor microenvironment have been optimizedby our research group; here, we continue to investigate the time-dependenteffect of CM exposure together with NKX3.1 changes, in which we observedthat these changes play important roles in gaining heterogeneous epithelial-to-mesenchymaltransition (EMT) phenotype. The gene discussed is NKX3-1; the disease is neoplasm.