Afterward, when vimentin expressionwas checked by western blotting, opposite results were obtained comparedto the previous N-cadherin data, where 6hNA cells showedhigh vimentin expression (Figure S1B).Previously, it has been shown in breast cancer samples that the coexpressionof E-cadherin and vimentin is associated with the most aggressivephenotype and poor prognosis; hence, increased E-cadherin expressionmay not always play roles in tumor suppression.33 Immunocytochemistry analysis proves the validity of vimentinexpression in subpopulations (Figure S1C). The gene discussed is VIM; the disease is breast carcinoma.