The present study reported that pediatric NAFLD is more likely to occur in older children and male children; pediatric NAFLD has clinical characteristics of elevated BMI and increased incidence of hypertension; pediatric NAFLD has alterations in biochemical characteristics of glucose and lipid metabolisms (low HDL-C, high TG, TC, LDL-C, and HbA1c), routine blood investigations (high WBC, NEU, LYM, MONO, RBC, and Hb), liver function tests (high ALT, AST, γ-GT, ALB, and GLB), and thyroid function tests (low FT3 and high TSH). This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.