Changes in surface marker expression were paralleled by large increases in cytokine production: IL-6 levels in response to PbSPZ-SAS(CL307) were increased 35-fold compared to wild-type PbSPZ (Figure 2D; 5,742 ± 1,391 vs. 166 ± 205 pg/mL, p < 0.0001), and increased 27-fold compared to both control PbSPZ-SAS (without adjuvant) (Figure 2D; 5,742 ± 1,391 vs. 211 ± 113 pg/mL, p < 0.0001) and to a cocktail of PbSPZ + concentration-matched soluble CL307 (Figure 2D; 5,742 ± 1,391 vs. 209 ± 206 pg/mL; p < 0.0001). Here, IL6 is linked to SATB2 associated disorder.