Given the lack of a murine ITI model that reflects the duration and intensity of ITI in patients, there are inherent limitations in the ability to assess the evolution of antibody development and elimination over time in murine models of hemophilia A. Moreover, this study evaluated antibody formation in naive hemophilia A mice injected with FVIII-IC in order to directly assess the impact of FVIII-IC epitope specificity. Here, F8 is linked to hemophilia A.