A LASSO regression analysis was performed on the 20 hub genes from the CRC patient cohort, and 9 characteristic genes associated with lymph node metastasis were screened, including KIF1A, TMEM59L, CLCA1, COL9A3, GDF5, TUBB2B, STMN2, FOXN1, and SCN5A (Figure 5(a)). This evidence concerns the gene CLCA1 and colorectal carcinoma.