Nine characteristic genes associated with lymph node metastasis were screened from the 20 hub genes using LASSO regression: KIF1A, TMEM59L, CLCA1, COL9A3, GDF5, TUBB2B, STMN2, FOXN1, and SCN5A. Here, TUBB2B is linked to metastatic malignant neoplasm in the lymph nodes.