As shown in Supplementary Figure 2, both ICAM1 ADCs demonstrated the same binding ability with their parent ICAM1 antibody in two human CCA cell lines (SK-ChA-1 and TFK-1), suggesting that conjugating ADC linkers and payloads on ICAM1 antibody via its inter-chain disulfide cysteine does not obviously affect its binding ability. Here, ICAM1 is linked to cholangiocarcinoma.