Furthermore, we performed immunofluorescent (IF) staining of ICAM1 on CCA cells, confirming that the overexpression of ICAM1 was localized on the plasma membranes of each CCA cell lines (HuCCT1, HCCC-9810, QBC939, SK-ChA-1, and TFK-1) but was absent on normal 293T cells (Fig. 1c), making it accessible for ICAM1 ADCs. This evidence concerns the gene ICAM1 and cholangiocarcinoma.