TOP1 and cancer: The reason we selected MC-VC-PAB-MMAE and MC-GGFG-DXd as our linker and payload combinations for ICAM1 ADC construction is due to the fact that they represent two different payload mechanism of actions (MOAs): MMAE works as a microtubule inhibitor suppressing cancer cell mitosis while DXd is a DNA topoisomerase I inhibitor and induces cancer cell death by generating DNA damages.