FUS-induced BBB opening has been shown to induce an inflammatory response in mice35–37, but no immunological response was observed at 7 days after FUS treatment in GBM patients in a previous reported clinical study20, Our study showed that only three differentially expressed genes, CX3CR1, HLA-DQB2, and MARCO, were related to the immune response in high-grade glioma tumors obtained at 1.7 ± 0.4 h post-FUS sonication. This evidence concerns the gene CX3CR1 and central nervous system cancer.