After establishing OIS-PRM in cultured cell models, we next tested it across two sets of archived FFPE HNSCC tumors: (1) 10 HPV(+) and 20 HPV(−) oropharyngeal squamous cell carcinomas collected as 50 μm curls (Fig. 5) and (2) punch biopsies from 19 HPV(−) oral squamous cell carcinomas including 11 NRF2 WT tumors and 8 tumors with NRF2E79Q or NRF2E79K activating mutations (Fig. 6). This evidence concerns the gene NFE2L2 and oral cavity squamous cell carcinoma.