P2RX7 and Alzheimer disease: Since the P2X7R antibody validated in vitro for use in western blot did not yield conclusive immunolabelling of human brain sections, RNAscope in situ hybridisation (ISH) was used as an orthogonal method to map the cell-specific distribution of P2RX7 mRNA in human control and AD brain in combination with immunostaining of neuronal and glial markers.