The rate of PBMC-derived CAR T cells with TSCM phenotype was significantly higher in both low and high TSCM groups following 48 h of co-culture with DSF/Cu+IR-stressed target breast cancer cells (SUM159) compared with B7-H3 CAR T cells co-cultured with untreated non-stressed SUM159 cells. The gene discussed is CD80; the disease is breast cancer.