SPON2 expression level based on MCPCOUNTER algorithm in BLCA (Rho = −0.825, P = 6.71e − 93), HNSC (Rho = 0.689, P = 1.96e − 70), HNSC-HPV + (Rho = 0.887, P = 5.36e − 31) and TGCT (Rho = 0.371, P = 3.64e − 06), XCELL algorithm in BRCA-LumA (Rho = 0.524, P = 9.15e − 38) and EPIC algorithm in CESC (Rho = 0.592, P = 1.23e − 27) and HNSC-HPV (Rho = 0.613, P = 1.12e − 42) were positively correlated with the number of cancer-associated fibroblast infiltration. The gene discussed is SPON2; the disease is cancer.