To identify the host proteases involved in viral entry, we first selected human cells that are susceptible to the infection of our SARS-CoV-2 Spike pseudotyped viral particles (Vpp), containing the Spike protein in the envelope and the luciferase gene inside, which can mimic the authentic SARS-CoV-2 viruses during the entry step of viral replication (6). Here, CHMP5 is linked to infection.