Thus, in order to investigate the effects of plasmin activity on NSCLC cell migration under basal conditions and after TGF-β stimulation, we detected cell migration in a boyden chamber assay applying three distinct protocols: I) 0.5% serum in both chambers, II) 0.5% in the upper and 10% serum in the lower chamber and III) 10% serum in both chambers (Fig. 6A, B). This evidence concerns the gene PLG and non-small cell lung carcinoma.