Flow cytometry and multiplex fluorescent immunohistochemistry (mIHC) further verified that RIME KO increased CD8+ T cells, IFN‐γ+ and granzyme B (GranB)+ CD8+ T cells in ESCC xenografts (Figure 5E–H and Figure S5E). The gene discussed is IFNG; the disease is esophageal squamous cell carcinoma.