CD4 and neoplasm: Figure 3 shows the cellular composition of the TME with and without prior ITI-3000 vaccination. Within treated tumors, there was a significant increase in the number of CD45+ hematopoietic cells and CD3+ T cells in comparison with control tumors (Figure 3A). Upon closer inspection of the CD45+CD3+ population, and in agreement with previous work, there was also an increase in the number of tumor-infiltrating CD4+ and CD8+ T cells from ITI-3000-treated animals, both in cells per gram tumor (Figure 3B) and as frequency of total CD45+ cells (Figure 3C).