We infected C57BL/6 mice intranasally with PR8, and 10 days after infection, we performed IFN-γ and IL-2 ELISpot assays with cells from individual spleens and pooled mediastinal lymph nodes (medLNs) stimulated with overlapping peptide pools spanning the whole protein (Tables S1, S2). This evidence concerns the gene IFNG and infection.