In line with our study, the efficacy of anti-CTLA-4 antibody was reported in other murine tumor models of colon cancer (ie., CT26, Colon 26, and MC38) which showed high sensitivity and durable responses to CTLA-4 blockade (Grosso and Jure-Kunkel, 2013; Wei et al., 2017; Sato et al., 2021). This evidence concerns the gene CTLA4 and colonic neoplasm.