In immunosuppressive mediator secretion, by comparing the expression of cytokines such as chemokines and receptors, interferons and receptors, and interleukins and receptors in the three subtypes most immunosuppressive mediators were lowly expressed in cluster 2, such as chemokines involved in T cell recruitment and inflammatory response: CXCL9 and CXCL10 were expressed at high levels in cluster 1, while the enrichment of CCL4 and CXCL13 may have promoted the cluster 1 establishment of the thermal tumor microenvironment46. The gene discussed is CCL4; the disease is neoplasm.