To confirm mutation of MMR genes and investigate the degree of TMB, we performed whole-exome sequencing (WES) on micro-dissected tumors at 16–20 weeks post-initiation, including 26 sgMsh2- and -3- control (sgCtl)-targeted KPC lung tumors, 15 and 6 SPC-Cre-targeted KPM and KP (Msh2 wild-type) lung tumors and 5 sgMsh2-, 6 sgMlh1-, 2 sgMsh6-, 6 sgMsh3- and 5 sgCtl-targeted colon tumors (Supplementary Table 1). Here, MSH2 is linked to colonic neoplasm.