Both vaccines as in previous studies9,19,23, were less efficient in inducing Wu-S-specific IFN-γ/TNF-α/IL-2 effector CD8 + T-cells (Fig. 4a) compared to the robust induction of CD4 + T-cell effectors (Fig. 3a) with significant induction noted only in CS seropositive subjects (Fig. 4a). Here, CD4 is linked to Cowden syndrome 1.