Our analysis found that AFP (AUROC 0.59 and 28.4% sensitivity, 90% specificity), AFP-L3 (AUROC 0.57 and 25.0% sensitivity, 90% specificity), PIVKA-II (AUROC 0.52 and 13.6% sensitivity, 90% specificity), and DCP (PIVKA-II) (AUROC 0.53 and 14.8% sensitivity, 90% specificity) did not discriminate pre-HCC from cirrhosis well. The gene discussed is ACE; the disease is Cirrhosis.