In the study, we found that PTEN expression was damaged in KIF18A knockout cells, but increased in KIF18A overexpression cells, which indicated that KIF18A can target PTEN to regulate CRC cells and also promote the activation of PI3K/Akt signaling pathway, leading to the upregulation of MMP2 and MMP9 expression. Here, MMP9 is linked to colorectal carcinoma.