TOX3 and colitis: To further investigate the role of TOX3-WDR5/ABCG2 signaling pathway in CRC stem-like traits maintenance and drug resistance development, we established a mouse model of colitis-associated colorectal tumorigenesis, in which mice were injected intraperitoneally with AOM, followed by 3 rounds of DSS treatment, and were treated with 0.9% NS or 2 mg/kg OXA qod, followed by first DSS and the development of colon tumors were examined after 80 days (Fig 6A).