In univariate analyses, male sex; the levels of ANA, IgG, and IgM; CAP value; and frequencies of CD4+ T cells, NK cells, Th1, Th17, CD4+ and CD8+ PD1+ T cells were significant determinants of AIH-overlap NASH (Table 4). This evidence concerns the gene CD40LG and metabolic dysfunction-associated steatohepatitis.