After defining each myeloid subcluster, we compared the cell ratio of each subcluster among 3 groups and found that Ma_3 was accumulated in atherosclerosis compared with NC (Figure 3F; Figure S5D), which was further validated by counting OPN (osteopontin; encoding gene SPP1) and CD68-positive cells using pathological staining pictures (Figure 3G). Here, CD68 is linked to atherosclerosis.