Importantly, a large study of 86 primary-recurrent patient-matched paired samples of IDH-WT GBM revealed a shift from proneural towards mesenchymal phenotypes using scRNA-seq.22 Through single-cell deconvolution of bulk RNA-seq, an analysis of 304 longitudinal patients demonstrated that IDH-Mut gliomas exhibit distinct cell-type changes during progression, tending to maintain a proneural phenotype and displaying an increase in stem-like neoplastic cells and a decrease in differentiated-like neoplastic cells.23 However, an analogous study at the single-cell level has not yet been undertaken. The gene discussed is IDH1; the disease is glioblastoma.