Cytotoxicity analysis was performed using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay of the ethanolic extract of TCF conducted on the MCF7 breast cancer cell line, and the bioactive compounds of TCF were further evaluated for their anticancer properties using MD, MDS, and binding energy calculation studies. Here, HNF4A is linked to breast carcinoma.