In this case report, we identified a novel non-reciprocal <i>ALK</i> fusion, <i>ALK-C2orf91</i>(intergenic) (A19: intergenic) and <i>PPFIA1</i>-<i>ALK</i> (P2:A20), by next-generation DNA sequencing in an advanced lung adenocarcinoma patient. Here, ALK is linked to lung adenocarcinoma.