The protein expression levels of MAP2K6 and E2F1 were significantly downregulated in PTTG3P or ILF3 knockdown NSCLC cells, and ILF3 knockdown could also significantly downregulate the mRNA and protein expression levels of MAP2K6 and E2F1 caused by overexpression of PTTG3P in NSCLC cells. The gene discussed is PTTG3P; the disease is non-small cell lung carcinoma.