We observed the expression level of genes in Module 2 (68 genes; eg. SLN, OSR2, ACTG2, RAMP1, ACTC1) were upregulated from the initial stages of the reprogramming trajectory and highly expressed at the pseudo-time endpoint, i.e. genes mainly expressed in adult patients with HF, were primarily involved in the regulation of muscle contraction and responses to zinc and copper ions (Figure 7H). This evidence concerns the gene ACTC1 and hydrops fetalis.