Genes included in Module 1 (321 genes, eg. ODC1, PFDN5, RPS3, RPLP0, RACK1) upregulated their expressions from the beginning and expressed high at the end of the pseudo-time trajectory, i.e. genes that were highly expressed in patients with HF, and were predominantly related to ribosome assembly and copper ion metabolism (Figure 5F-G). The gene discussed is RPS3; the disease is hydrops fetalis.