Univariate Cox regression analysis showed that ALT, GGT, AFP, tumor size, tumor encapsulation, macroscopic tumor thrombus, microvascular invasion (MVI), lymphatic metastasis, differentiation grade, BCLC stage and PGAM1 expression were significantly correlated with the survival of HCC patients (Figure S1G and Table S1, Supporting Information). The gene discussed is AFP; the disease is hepatocellular carcinoma.