First, we confirmed that knockdown of PGAM1 in the PLC/PRF/5 and SNU398 human HCC cell lines and Hepa1‐6 mouse HCC cell lines significantly decreased cell proliferation, while overexpression of PGAM1 in the Hep3B human HCC cell line significantly increased cell proliferation (Figure 3E; Figure S3D, Supporting Information). This evidence concerns the gene PGAM1 and hepatocellular carcinoma.