PGAM1 was initially considered a critical metabolic enzyme involved in glycolysis and biosynthesis, but its inhibition has been gradually acknowledged to cosuppress several metabolic and cancerous pathways.[6] To illustrate the potential mechanism by which PGAM1‐mediated metabolic remodeling contributes to HCC progression, we performed transcriptomic and untargeted metabolomic analysis of shNC and shPgam1 Hepa16 subcutaneous tumors in C57BL/6 mice (n = 3 per group). Here, PGAM1 is linked to hepatocellular carcinoma.