Consistent with the RNA‐Seq results based on different subcellular localizations, we performed a subcellular separation assay and FISH analysis in bladder cancer tissues and cell lines, and found that lncRNA BCCE4 was mostly enriched in the cytoplasm (Figures 2D and 3G,H and; Figure S16, Supporting Information). This evidence concerns the gene EMSLR and urinary bladder carcinoma.