GAPDH and RPL32 mRNAs are widely used as references for qPCR assays in other mammalian cells such as adipocytes and macrophages [28, 29, 35, 36], but GAPDH and RPL32 mRNA levels had much larger standard deviations (ΔCT was 3.15 and 3.10, respectively) and the most abundantly expressed with 15.5- and 16.4-fold of BCL2 mRNA, respectively in the human colon cancer cells (Table 1). The gene discussed is GAPDH; the disease is malignant colon neoplasm.